WNT10A Polyclonal Antibody (CAB15602)

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CAB15602
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Description

Antibody DatasheetAntibody MSDS

WNT10A Polyclonal Antibody (CAB15602)

Overview

Title:WNT10A Polyclonal Antibody (CAB15602)
Size:100µL
Code:CAB15602
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 158-417 of human WNT10A (NP_079492.2).
GeneID:80326
SwissProt:Q9GZT5
Synonyms:WNT10A; OODD; SSPS; STHAG4; protein Wnt-10a
Calculated MW:46kDa
Observed MW:46kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:Mouse brain, Rat brain
Cellular Location:Secreted, extracellular matrix, extracellular space

Antibody Images

Western blot - WNT10A Polyclonal Antibody (CAB15602)

Western blot analysis of extracts of various cell lines, using WNT10A antibody (CAB15602) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

WNT10A Polyclonal Antibody (CAB15602) Protein Information

UniProt Protein Function:WNT10A: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Defects in WNT10A are a cause of ectodermal dysplasia anhidrotic (EDA); also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. Most patients carrying WNT10A mutations present with sweating anomalies. However, comparison with EDA cases harboring mutations in the ectodysplasin pathway identifies some phenotypic differences. Dermatological features (anomalies of hair and sweat glands) are less severe in patients carrying WNT10A mutations and facial dysmorphism can be absent. The dental phenotype consists in microdontia, whereas teeth agenesis is more frequent in patients carrying mutations in the ectodysplasin pathway. Defects in WNT10A are a cause of odonto-onycho-dermal dysplasia (OODD). OODD is a rare autosomal recessive ectodermal dysplasia in which the presenting phenotype is dry hair, severe hypodontia, smooth tongue with marked reduction of fungiform and filiform papillae, onychodysplasia, keratoderma and hyperhidrosis of palms and soles, and hyperkeratosis of the skin. Defects in WNT10A are a cause of Schopf-Schulz-Passarge syndrome (SSPS). SSPS is rare ectodermal dysplasia, characterized chiefly by cysts of the eyelid margins, palmoplantar keratoderma, hypodontia, hypotrichosis and nail dystrophy. Multiple eyelid apocrine hidrocystomas are the hallmark of this condition, although they usually appear in adulthood. The concomitant presence of eccrine syringofibroadenoma in most patients and of other adnexal skin tumors in some affected subjects indicates that Schopf-Schulz-Passarge syndrome is a genodermatosis with skin appendage neoplasms. Belongs to the Wnt family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function:frizzled binding

Biological Process: skin development; neuron differentiation; odontogenesis; tongue development; hair follicle morphogenesis; Wnt receptor signaling pathway; cell fate commitment; hair follicle development; epidermis morphogenesis; sebaceous gland development; neural crest cell differentiation; regulation of odontogenesis of dentine-containing teeth

Disease: Schopf-schulz-passarge Syndrome; Tooth Agenesis, Selective, 4; Odontoonychodermal Dysplasia

NCBI Summary:The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region. [provided by RefSeq, Jul 2008]
UniProt Code:Q9GZT5
NCBI GenInfo Identifier:14424011
NCBI Gene ID:80326
NCBI Accession:Q9GZT5.1
UniProt Secondary Accession:Q9GZT5,Q53S44, Q96TA7, Q9H7S8,
UniProt Related Accession:Q9GZT5
Molecular Weight:46,444 Da
NCBI Full Name:Protein Wnt-10a
NCBI Synonym Full Names:wingless-type MMTV integration site family, member 10A
NCBI Official Symbol:WNT10A  
NCBI Official Synonym Symbols:OODD; SSPS; STHAG4  
NCBI Protein Information:protein Wnt-10a
UniProt Protein Name:Protein Wnt-10a
UniProt Gene Name:WNT10A  
UniProt Entry Name:WN10A_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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