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WIPI1 Polyclonal Antibody (CAB7528)

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SKU:
CAB7528
  • Western blot - WIPI1 Polyclonal Antibody (CAB7528)
  • Immunohistochemistry - WIPI1 Polyclonal Antibody (CAB7528)
€299.00

Description

Overview

Title:WIPI1 Polyclonal Antibody (CAB7528)
Size:100µL
Code:CAB7528
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 187-446 of human WIPI1 (NP_060453.3).
GeneID:55062
SwissProt:Q5MNZ9
Synonyms:WIPI1; ATG18; ATG18A; WIPI49; WD repeat domain, phosphoinositide interacting 1
Calculated MW:48kDa
Observed MW:46kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:SW480, BxPC-3, Mouse eye, Rat brain, Rat eye
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endosome, Golgi apparatus, Peripheral membrane protein, Preautophagosomal structure membrane, clathrin-coated vesicle, cytoskeleton, trans-Golgi network

Antibody Images

Western blot - WIPI1 Polyclonal Antibody (CAB7528)

Western blot analysis of extracts of various cell lines, using WIPI1 antibody (CAB7528) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Immunohistochemistry - WIPI1 Polyclonal Antibody (CAB7528)

Immunohistochemistry of paraffin-embedded human brain cancer using WIPI1 Antibody (CAB7528) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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