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ULk1 Polyclonal Antibody (CAB8529)

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SKU:
CAB8529
  • Western blot - ULk1 Polyclonal Antibody (CAB8529)
  • Immunohistochemistry - ULk1 Polyclonal Antibody (CAB8529)
  • Immunohistochemistry - ULk1 Polyclonal Antibody (CAB8529)
  • Immunofluorescence - ULk1 Polyclonal Antibody (CAB8529)
€299.00

Description

Overview

Title:ULk1 Polyclonal Antibody (CAB8529)
Size:100µL
Code:CAB8529
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:A synthetic peptide of human ULk1
GeneID:8408
SwissProt:O75385
Synonyms:ULK1; ATG1; ATG1A; UNC51; Unc51.1; hATG1; serine/threonine-protein kinase ULK1
Calculated MW:112kDa
Observed MW:150kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:1000; IHC 1:50 - 1:200; IF 1:10 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:293T, HeLa
Cellular Location:Cytoplasm, Preautophagosomal structure, cytosol

Antibody Images

Western blot - ULk1 Polyclonal Antibody (CAB8529)

Western blot analysis of extracts of various cell lines, using ULK1 antibody (CAB8529) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Immunohistochemistry - ULk1 Polyclonal Antibody (CAB8529)

Immunohistochemistry of paraffin-embedded mouse liver using ULk1 antibody (CAB8529) at dilution of 1:100 (40x lens).

Immunohistochemistry - ULk1 Polyclonal Antibody (CAB8529)

Immunohistochemistry of paraffin-embedded mouse heart using ULk1 antibody (CAB8529) at dilution of 1:100 (40x lens).

Immunofluorescence - ULk1 Polyclonal Antibody (CAB8529)

Immunofluorescence analysis of A549 cells using ULk1 antibody (CAB8529).Green: ULK-1 protein stained by anti-ULK-1 antibody diluted at 1:300. Red: alpha-tubulin stained by monoclonal anti-tubulin antibody diluted at 1:100. Scale bar = 10 ?m.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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