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TUBA4A Polyclonal Antibody (CABC031)

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SKU:
CABC031
$331.89

Description

Overview

Title:TUBA4A Polyclonal Antibody (CABC031)
Size:100uL
Code:CABC031
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant protein of human TUBA4A
GeneID:7277
SwissProt:P68366
Synonyms:TUBA4A; ALS22; H2-ALPHA; TUBA1; tubulin alpha 4a
Calculated MW:48kDa/49kDa
Observed MW:55kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC;
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:HeLa, Jurkat, A-431, Mouse brain, Mouse lung, Rat brain
Cellular Location:Cytoplasm, cytoskeleton

Antibody Images

Western blot - TUBA4A Polyclonal Antibody (CABC031)

Western blot analysis of extracts of various cell lines, using TUBA4A antibody (CABC031) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
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Additional Information

ANTIBODY TYPE:
Polyclonal
APPLICATION:
WB
APPLICATION:
IHC
REACTIVITY:
Human
REACTIVITY:
Mouse
REACTIVITY:
Rat
HOST SPECIES:
Rabbit
ISOTYPE:
IgG
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