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TriMethyl-Histone H3-K27 mAb (CAB16199)

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CAB16199
  • Western blot - TriMethyl-Histone H3-K27 mAb (CAB16199)
  • - TriMethyl-Histone H3-K27 mAb (CAB16199)
  • Immunohistochemistry - TriMethyl-Histone H3-K27 mAb (CAB16199)
  • Immunohistochemistry - TriMethyl-Histone H3-K27 mAb (CAB16199)
  • Immunohistochemistry - TriMethyl-Histone H3-K27 mAb (CAB16199)
  • Immunofluorescence - TriMethyl-Histone H3-K27 mAb (CAB16199)
  • Immunofluorescence - TriMethyl-Histone H3-K27 mAb (CAB16199)
  • Immunofluorescence - TriMethyl-Histone H3-K27 mAb (CAB16199)
$560.55

Description

Overview

Title:TriMethyl-Histone H3-K27 mAb (CAB16199)
Size:100µL
Code:CAB16199
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen Information

Immunogen:A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
GeneID:8290
SwissProt:Q16695
Synonyms:HIST3H3; H3.4; H3/g; H3FT; H3t; histone H3.1t
Calculated MW:15kDa
Observed MW:Refer to figures

Applications

Reactivity:Human
Tested Applications:WB; IHC; IF; IP; ChIP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:200; ChIP 1:20 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:N/A
Cellular Location:Chromosome, Nucleus

Antibody Images

Western blot - TriMethyl-Histone H3-K27 mAb (CAB16199)

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (CAB16199) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

 - TriMethyl-Histone H3-K27 mAb (CAB16199)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (CAB16199) at 1:1000 dilution.

Immunohistochemistry - TriMethyl-Histone H3-K27 mAb (CAB16199)

Immunohistochemistry of paraffin-embedded human colon carcinoma using TriMethyl-Histone H3-K27 antibody (CAB16199) at dilution of 1:25 (40x lens).

Immunohistochemistry - TriMethyl-Histone H3-K27 mAb (CAB16199)

Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (CAB16199) at dilution of 1:25 (40x lens).

Immunohistochemistry - TriMethyl-Histone H3-K27 mAb (CAB16199)

Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K27 antibody (CAB16199) at dilution of 1:25 (40x lens).

Immunofluorescence - TriMethyl-Histone H3-K27 mAb (CAB16199)

Immunofluorescence analysis of C6 cells using TriMethyl-Histone H3-K27 antibody (CAB16199) at dilution of 1:25. Blue: DAPI for nuclear staining.

Immunofluorescence - TriMethyl-Histone H3-K27 mAb (CAB16199)

Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K27 antibody (CAB16199) at dilution of 1:25. Blue: DAPI for nuclear staining.

Immunofluorescence - TriMethyl-Histone H3-K27 mAb (CAB16199)

Immunofluorescence analysis of U-2OS cells using TriMethyl-Histone H3-K27 antibody (CAB16199) at dilution of 1:25. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Application:
ChIP
Reactivity:
Human
Host Species:
Mouse
Isotype:
IgG
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