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TH Polyclonal Antibody (CAB0028)

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CAB0028
  • Western blot - TH Polyclonal Antibody (CAB0028)
  • Immunohistochemistry - TH Polyclonal Antibody (CAB0028)
  • Immunohistochemistry - TH Polyclonal Antibody (CAB0028)
€299.00

Description

Overview

Title:TH Polyclonal Antibody (CAB0028)
Size:100µL
Code:CAB0028
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TH (NP_954986.2).
GeneID:7054
SwissProt:P07101
Synonyms:TH; DYT14; DYT5b; TYH; tyrosine hydroxylase
Calculated MW:44kDa/45kDa/55kDa/56kDa/58kDa
Observed MW:60kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:1000; IHC 1:50 - 1:100; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:Mouse brain, Rat brain
Cellular Location:N/A

Antibody Images

Western blot - TH Polyclonal Antibody (CAB0028)

Western blot analysis of extracts of various cell lines, using TH antibody (CAB0028) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Immunohistochemistry - TH Polyclonal Antibody (CAB0028)

Immunohistochemistry of paraffin-embedded rat brain using TH Antibody (CAB0028) at dilution of 1:100 (40x lens).

Immunohistochemistry - TH Polyclonal Antibody (CAB0028)

Immunohistochemistry of paraffin-embedded mouse spinal cord using TH Antibody (CAB0028) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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