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SQSTM1 Polyclonal Antibody (CAB11250)

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CAB11250
  • Western blot - SQSTM1 Polyclonal Antibody (CAB11250)
  • Immunohistochemistry - SQSTM1 Polyclonal Antibody (CAB11250)
  • Immunohistochemistry - SQSTM1 Polyclonal Antibody (CAB11250)
  • Immunohistochemistry - SQSTM1 Polyclonal Antibody (CAB11250)
  • Immunohistochemistry - SQSTM1 Polyclonal Antibody (CAB11250)
  • Immunofluorescence - SQSTM1 Polyclonal Antibody (CAB11250)
  • Immunofluorescence - SQSTM1 Polyclonal Antibody (CAB11250)
€299.00

Description

Overview

Title:SQSTM1 Polyclonal Antibody (CAB11250)
Size:100µL
Code:CAB11250
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 (NP_001135770.1).
GeneID:8878
SwissProt:Q13501
Synonyms:SQSTM1; A170; DMRV; FTDALS3; NADGP; OSIL; PDB3; ZIP3; p60; p62; p62B; sequestosome-1
Calculated MW:38kDa/47kDa
Observed MW:62kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:H460, HeLa, Jurkat, Mouse heart, Mouse liver, Mouse kidney, Rat liver
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome

Antibody Images

Western blot - SQSTM1 Polyclonal Antibody (CAB11250)

Western blot analysis of extracts of various cell lines, using SQSTM1 antibody (CAB11250) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunohistochemistry - SQSTM1 Polyclonal Antibody (CAB11250)

Immunohistochemistry of paraffin-embedded human tonsil using SQSTM1 antibody (CAB11250) at dilution of 1:100 (40x lens).

Immunohistochemistry - SQSTM1 Polyclonal Antibody (CAB11250)

Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1 antibody (CAB11250) at dilution of 1:100 (40x lens).

Immunohistochemistry - SQSTM1 Polyclonal Antibody (CAB11250)

Immunohistochemistry of paraffin-embedded human tonsil using P62 antibody (CAB11250) at dilution of 1:150 (40x lens).

Immunohistochemistry - SQSTM1 Polyclonal Antibody (CAB11250)

Immunohistochemistry of paraffin-embedded human colon carcinoma using P62 antibody (CAB11250) at dilution of 1:150 (40x lens).

Immunofluorescence - SQSTM1 Polyclonal Antibody (CAB11250)

Immunofluorescence analysis of HeLa cells using P62 antibody (CAB11250) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - SQSTM1 Polyclonal Antibody (CAB11250)

Immunofluorescence analysis of NIH/3T3 cells using P62 antibody (CAB11250) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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