SH3GLB1 Polyclonal Antibody (CAB14887)

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CAB14887
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Description

Antibody DatasheetAntibody MSDS

SH3GLB1 Polyclonal Antibody (CAB14887)

Overview

Title:SH3GLB1 Polyclonal Antibody (CAB14887)
Size:100µL
Code:CAB14887
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-365 of human SH3GLB1 (NP_057093.1).
GeneID:51100
SwissProt:Q9Y371
Synonyms:SH3GLB1; Bif-1; CGI-61; PPP1R70; dJ612B15.2; endophilin-B1
Calculated MW:29kDa/40kDa/43kDa
Observed MW:41kDa

Applications

Reactivity:Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:Mouse testis, Rat testis
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Golgi apparatus membrane, Midbody, Mitochondrion outer membrane, Peripheral membrane protein, autophagosome membrane

Antibody Images

Western blot - SH3GLB1 Polyclonal Antibody (CAB14887)

Western blot analysis of extracts of various cell lines, using SH3GLB1 antibody (CAB14887) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.

SH3GLB1 Polyclonal Antibody (CAB14887) Protein Information

UniProt Protein Function:SH3GLB1: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1. Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding. Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. Belongs to the endophilin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial; Apoptosis; EC 2.3.1.-; Autophagy; Transferase

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: cytoplasm; midbody

Molecular Function:identical protein binding; protein binding; protein homodimerization activity

Biological Process: autophagic cell death; cellular response to amino acid starvation; cellular response to glucose starvation; positive regulation of autophagy; positive regulation of protein oligomerization; protein oligomerization; receptor catabolic process; regulation of cytokinesis; regulation of macroautophagy; regulation of protein stability

NCBI Summary:This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
UniProt Code:Q9Y371
NCBI GenInfo Identifier:41018158
NCBI Gene ID:51100
NCBI Accession:Q9Y371.1
UniProt Secondary Accession:Q9Y371,Q5H8U5, Q9H3Z0, Q9NR47, Q9NYA9, B4E182,
UniProt Related Accession:Q9Y371
Molecular Weight:29,318 Da
NCBI Full Name:Endophilin-B1
NCBI Synonym Full Names:SH3 domain containing GRB2 like endophilin B1
NCBI Official Symbol:SH3GLB1  
NCBI Official Synonym Symbols:Bif-1; CGI-61; PPP1R70; dJ612B15.2  
NCBI Protein Information:endophilin-B1
UniProt Protein Name:Endophilin-B1
UniProt Synonym Protein Names:Bax-interacting factor 1; Bif-1; SH3 domain-containing GRB2-like protein B1
Protein Family:Endophilin
UniProt Gene Name:SH3GLB1  
UniProt Entry Name:SHLB1_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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