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RPA1 Polyclonal Antibody (CAB0874)

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SKU:
CAB0874
$331.89

Description

Overview

Title:RPA1 Polyclonal Antibody (CAB0874)
Size:100µL
Code:CAB0874
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RPA1 (NP_002936.1).
GeneID:6117
SwissProt:P27694
Synonyms:RPA1; HSSB; MST075; REPA1; RF-A; RP-A; RPA70; replication protein A1
Calculated MW:68kDa
Observed MW:70kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:U-87MG, HeLa, 293T, A-549
Cellular Location:Nucleus, PML body

Antibody Images

Western blot - RPA1 Polyclonal Antibody (CAB0874)

Western blot analysis of extracts of various cell lines, using RPA1 antibody (CAB0874) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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