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Rabbit anti DDDDK-Tag mAb (CABE063)

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SKU:
CABE063
  • Western blot - Rabbit anti DDDDK-Tag mAb (CABE063)
  • Immunofluorescence - Rabbit anti DDDDK-Tag mAb (CABE063)
  • Immunoprecipitation - Rabbit anti DDDDK-Tag mAb (CABE063)
€399.00

Description

Overview

Title:Rabbit anti DDDDK-Tag mAb (CABE063)
Size:100 uL
Code:CABE063
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:A synthetic Peptide of human DDDDK-Tag
GeneID:N/A
SwissProt:N/A
Synonyms:N/A
Calculated MW:N/A
Observed MW:N/A

Applications

Reactivity:N/A
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:2000 - 1:5000; IHC 1:50 - 1:500; IF 1:50 - 1:500; IP 1:50 - 1:500
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:293T, HeLa
Cellular Location:N/A

Antibody Images

Western blot - Rabbit anti DDDDK-Tag mAb (CABE063)

Western blot analysis of extracts of over-expressed DDDDK-tagged protein in 293T cells, using Rabbit anti DDDDK-Tag mAb (CABE063) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunofluorescence - Rabbit anti DDDDK-Tag mAb (CABE063)

Immunofluorescence analysis of GFP-DDDDK transgenic HeLa cells using DDDDK-Tag antibody (CABE063). Green: GFP expression. Blue: DAPI for nuclear staining. 

Immunoprecipitation - Rabbit anti DDDDK-Tag mAb (CABE063)

Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (CABE063). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Host Species:
Rabbit
Isotype:
IgG
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