RAB7A Polyclonal Antibody (CAB12344)

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CAB12344
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Description

Antibody DatasheetAntibody MSDS

RAB7A Polyclonal Antibody (CAB12344)

Overview

Title:RAB7A Polyclonal Antibody (CAB12344)
Size:100µL
Code:CAB12344
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

Immunogen Information

Immunogen:Recombinant protein of human RAB7A
GeneID:7879
SwissProt:P51149
Synonyms:RAB7A; PRO2706; RAB7; ras-related protein Rab-7a
Calculated MW:23kDa
Observed MW:23kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; FC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100; FC 1:20 - 1:50
Storage Buffer:Store at 4°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Flow Cytometry    
Positive Samples:A-431, HeLa
Cellular Location:Cytoplasmic side, Cytoplasmic vesicle, Late endosome membrane, Lipid droplet, Lysosome membrane, Melanosome membrane, Peripheral membrane protein, autophagosome membrane, phagosome membrane

Antibody Images

Western blot - RAB7A Polyclonal Antibody (CAB12344)

Western blot analysis of extracts of various cell lines, using RAB7A antibody (CAB12344).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

RAB7A Polyclonal Antibody (CAB12344) Protein Information

UniProt Protein Function:Rab7: a member of the RAB family of RAS-related GTP-binding proteins, important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori. Mutations in this protein cause Charcot-Marie-Tooth type 2B neuropathy.
UniProt Protein Details:

Protein type:G protein, monomeric; G protein, monomeric, Rab; G protein

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; intracellular membrane-bound organelle; lysosome; retromer complex; lysosomal membrane; late endosome; vacuolar membrane; pre-autophagosomal structure membrane; melanosome; phagocytic vesicle

Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; Rac GTPase binding

Biological Process: protein transport; protein targeting to lysosome; early endosome to late endosome transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; endosome to lysosome transport; endocytosis; protein to membrane docking; Rab protein signal transduction; retrograde transport, endosome to Golgi; epidermal growth factor ligand processing; bone resorption

Disease: Charcot-marie-tooth Disease, Axonal, Type 2b

NCBI Summary:RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies. [provided by RefSeq, Jul 2008]
UniProt Code:P51149
NCBI GenInfo Identifier:1709999
NCBI Gene ID:7879
NCBI Accession:P51149.1
UniProt Related Accession:P51149
Molecular Weight:
NCBI Full Name:Ras-related protein Rab-7a
NCBI Synonym Full Names:RAB7A, member RAS oncogene family
NCBI Official Symbol:RAB7A  
NCBI Official Synonym Symbols:RAB7; CMT2B; PRO2706  
NCBI Protein Information:ras-related protein Rab-7a
UniProt Protein Name:Ras-related protein Rab-7a
Protein Family:Ras-related protein
UniProt Gene Name:RAB7A  
UniProt Entry Name:RAB7A_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
FC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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