PYCARD Polyclonal Antibody (CAB16672)

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CAB16672
  • Western blot - PYCARD Polyclonal Antibody (CAB16672)
  • Immunohistochemistry - PYCARD Polyclonal Antibody (CAB16672)
  • Immunoprecipitation - PYCARD Polyclonal Antibody (CAB16672)
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Description

Antibody DatasheetAntibody MSDS

PYCARD Polyclonal Antibody (CAB16672)

Overview

Title:PYCARD Polyclonal Antibody (CAB16672)
Size:100µL
Code:CAB16672
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant protein of human PYCARD.
GeneID:29108
SwissProt:Q9ULZ3
Synonyms:PYCARD; ASC; CARD5; TMS; TMS-1; TMS1; PYD and CARD domain containing
Calculated MW:15kDa/19kDa/21kDa
Observed MW:25kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:HT-29, BT-474, U-937, mouse thymus, mouse spleen, rat spleen
Cellular Location:Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus

Antibody Images

Western blot - PYCARD Polyclonal Antibody (CAB16672)

Western blot analysis of extracts of various cell lines, using PYCARD antibody (CAB16672) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - PYCARD Polyclonal Antibody (CAB16672)

Immunohistochemistry of paraffin-embedded human placenta using PYCARD antibody (CAB16672) at dilution of 1:100 (40x lens).

Immunoprecipitation - PYCARD Polyclonal Antibody (CAB16672)

Immunoprecipitation analysis of 200ug extracts of U937 cells using 3ug PYCARD antibody (CAB16672). Western blot was performed from the immunoprecipitate using PYCARD antibody (CAB16672) at a dilition of 1:1000.

PYCARD Polyclonal Antibody (CAB16672) Protein Information

UniProt Protein Function:PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell soma; mitochondrion; endoplasmic reticulum; cytoplasm; nucleolus; extracellular region; IkappaB kinase complex; cytosol; nucleus

Molecular Function:protein binding; protein homodimerization activity; protease binding; caspase activator activity; Pyrin domain binding

Biological Process: myeloid dendritic cell activation during immune response; apoptosis; positive regulation of apoptosis; regulation of protein stability; positive regulation of caspase activity; positive regulation of JNK cascade; positive regulation of interleukin-1 beta secretion; positive regulation of activated T cell proliferation; signal transduction; defense response to Gram-negative bacterium; activation of NF-kappaB transcription factor; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; inflammatory response; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus; positive regulation of adaptive immune response; caspase activation; interleukin-1 beta production; positive regulation of interleukin-6 production; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of tumor necrosis factor production; negative regulation of interferon-beta production; myeloid dendritic cell activation; positive regulation of interferon-gamma production; positive regulation of actin filament polymerization; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; innate immune response; regulation of autophagy; positive regulation of transcription factor activity; positive regulation of T cell activation; positive regulation of defense response to virus by host; activation of innate immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II

NCBI Summary:This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9ULZ3
NCBI GenInfo Identifier:18203507
NCBI Gene ID:29108
NCBI Accession:Q9ULZ3.2
UniProt Related Accession:Q9ULZ3
Molecular Weight:21 KD
NCBI Full Name:Apoptosis-associated speck-like protein containing a CARD
NCBI Synonym Full Names:PYD and CARD domain containing
NCBI Official Symbol:PYCARD  
NCBI Official Synonym Symbols:ASC; TMS; TMS1; CARD5; TMS-1  
NCBI Protein Information:apoptosis-associated speck-like protein containing a CARD
UniProt Protein Name:Apoptosis-associated speck-like protein containing a CARD
UniProt Synonym Protein Names:Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1
Protein Family:Ascalin
UniProt Gene Name:PYCARD  
UniProt Entry Name:ASC_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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