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PYCARD Polyclonal Antibody (CAB16672)

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CAB16672
  • Western blot - PYCARD Polyclonal Antibody (CAB16672)
  • Immunohistochemistry - PYCARD Polyclonal Antibody (CAB16672)
  • Immunoprecipitation - PYCARD Polyclonal Antibody (CAB16672)
$331.89

Description

Overview

Title:PYCARD Polyclonal Antibody (CAB16672)
Size:100µL
Code:CAB16672
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant protein of human PYCARD.
GeneID:29108
SwissProt:Q9ULZ3
Synonyms:PYCARD; ASC; CARD5; TMS; TMS-1; TMS1; PYD and CARD domain containing
Calculated MW:15kDa/19kDa/21kDa
Observed MW:25kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:HT-29, BT-474, U-937, mouse thymus, mouse spleen, rat spleen
Cellular Location:Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus

Antibody Images

Western blot - PYCARD Polyclonal Antibody (CAB16672)

Western blot analysis of extracts of various cell lines, using PYCARD antibody (CAB16672) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - PYCARD Polyclonal Antibody (CAB16672)

Immunohistochemistry of paraffin-embedded human placenta using PYCARD antibody (CAB16672) at dilution of 1:100 (40x lens).

Immunoprecipitation - PYCARD Polyclonal Antibody (CAB16672)

Immunoprecipitation analysis of 200ug extracts of U937 cells using 3ug PYCARD antibody (CAB16672). Western blot was performed from the immunoprecipitate using PYCARD antibody (CAB16672) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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