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PUF60 Polyclonal Antibody (CAB6709)

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SKU:
CAB6709
  • Western blot - PUF60 Polyclonal Antibody (CAB6709)
  • Immunohistochemistry - PUF60 Polyclonal Antibody (CAB6709)
  • Immunofluorescence - PUF60 Polyclonal Antibody (CAB6709)
  • Immunoprecipitation - PUF60 Polyclonal Antibody (CAB6709)
$331.89

Description

Overview

Title:PUF60 Polyclonal Antibody (CAB6709)
Size:100µL
Code:CAB6709
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2).
GeneID:22827
SwissProt:Q9UHX1
Synonyms:PUF60; FIR; RoBPI; SIAHBP1; VRJS; poly(U) binding splicing factor 60
Calculated MW:54kDa/55kDa/57kDa/58kDa/59kDa
Observed MW:65kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:100; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:THP-1, A-549, Mouse brain, Mouse thymus, Mouse spleen, Rat lung, Rat liver, Rat heart
Cellular Location:Nucleus

Antibody Images

Western blot - PUF60 Polyclonal Antibody (CAB6709)

Western blot analysis of extracts of various cell lines, using PUF60 antibody (CAB6709) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - PUF60 Polyclonal Antibody (CAB6709)

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PUF60 antibody (CAB6709) at dilution of 1:100 (40x lens).

Immunofluorescence - PUF60 Polyclonal Antibody (CAB6709)

Immunofluorescence analysis of HeLa cells using PUF60 antibody (CAB6709). Blue: DAPI for nuclear staining.

Immunoprecipitation - PUF60 Polyclonal Antibody (CAB6709)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug PUF60 antibody (CAB6709). Western blot was performed from the immunoprecipitate using PUF60 antibody (CAB6709) at a dilition of 1:500.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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