PUF60 Polyclonal Antibody (CAB6709)

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CAB6709
  • Western blot - PUF60 Polyclonal Antibody (CAB6709)
  • Immunohistochemistry - PUF60 Polyclonal Antibody (CAB6709)
  • Immunofluorescence - PUF60 Polyclonal Antibody (CAB6709)
  • Immunoprecipitation - PUF60 Polyclonal Antibody (CAB6709)
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Description

Antibody DatasheetAntibody MSDS

PUF60 Polyclonal Antibody (CAB6709)

Overview

Title:PUF60 Polyclonal Antibody (CAB6709)
Size:100µL
Code:CAB6709
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2).
GeneID:22827
SwissProt:Q9UHX1
Synonyms:PUF60; FIR; RoBPI; SIAHBP1; VRJS; poly(U) binding splicing factor 60
Calculated MW:54kDa/55kDa/57kDa/58kDa/59kDa
Observed MW:65kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:100; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:THP-1, A-549, Mouse brain, Mouse thymus, Mouse spleen, Rat lung, Rat liver, Rat heart
Cellular Location:Nucleus

Antibody Images

Western blot - PUF60 Polyclonal Antibody (CAB6709)

Western blot analysis of extracts of various cell lines, using PUF60 antibody (CAB6709) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunohistochemistry - PUF60 Polyclonal Antibody (CAB6709)

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PUF60 antibody (CAB6709) at dilution of 1:100 (40x lens).

Immunofluorescence - PUF60 Polyclonal Antibody (CAB6709)

Immunofluorescence analysis of HeLa cells using PUF60 antibody (CAB6709). Blue: DAPI for nuclear staining.

Immunoprecipitation - PUF60 Polyclonal Antibody (CAB6709)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug PUF60 antibody (CAB6709). Western blot was performed from the immunoprecipitate using PUF60 antibody (CAB6709) at a dilition of 1:500.

PUF60 Polyclonal Antibody (CAB6709) Protein Information

UniProt Protein Function:FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:RNA splicing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; Golgi apparatus; mitochondrion; ribonucleoprotein complex; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function:identical protein binding; protein binding; DNA binding; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; RNA splicing; mRNA processing

Disease: Verheij Syndrome

NCBI Summary:This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
UniProt Code:Q9UHX1
NCBI GenInfo Identifier:74761960
NCBI Gene ID:22827
NCBI Accession:Q9UHX1.1
UniProt Secondary Accession:Q9UHX1,Q969E7, Q96D94, Q96H63, Q99628, Q9NZA0, Q9UJY7 A8K8K8,
UniProt Related Accession:Q9UHX1
Molecular Weight:559
NCBI Full Name:Poly(U)-binding-splicing factor PUF60
NCBI Synonym Full Names:poly-U binding splicing factor 60KDa
NCBI Official Symbol:PUF60  
NCBI Official Synonym Symbols:FIR; VRJS; RoBPI; SIAHBP1  
NCBI Protein Information:poly(U)-binding-splicing factor PUF60; poly(U)-binding-splicing factor PUF60; Siah binding protein 1; FBP interacting repressor; Ro ribonucleoprotein-binding protein 1; pyrimidine tract binding splicing factor; FUSE-binding protein-interacting repressor
UniProt Protein Name:Poly(U)-binding-splicing factor PUF60
UniProt Synonym Protein Names:60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1
Protein Family:Poly(U)-binding-splicing factor
UniProt Gene Name:PUF60  
UniProt Entry Name:PUF60_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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