PRKCD Polyclonal Antibody (CAB0471)

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CAB0471
  • Western blot - PRKCD Polyclonal Antibody (CAB0471)
  • Immunohistochemistry - PRKCD Polyclonal Antibody (CAB0471)
  • Immunohistochemistry - PRKCD Polyclonal Antibody (CAB0471)
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Description

Antibody DatasheetAntibody MSDS

PRKCD Polyclonal Antibody (CAB0471)

Overview

Title:PRKCD Polyclonal Antibody (CAB0471)
Size:100µL
Code:CAB0471
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 477-676 of human PRKCD (NP_997704.1).
GeneID:5580
SwissProt:Q05655
Synonyms:PRKCD; ALPS3; CVID9; MAY1; PKCD; nPKC-delta; protein kinase C delta
Calculated MW:77kDa/80kDa
Observed MW:78kDa/85kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:MCF7, Mouse testis
Cellular Location:Cell membrane, Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, Peripheral membrane protein, perinuclear region

Antibody Images

Western blot - PRKCD Polyclonal Antibody (CAB0471)

Western blot analysis of extracts of various cell lines, using PRKCD antibody (CAB0471) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - PRKCD Polyclonal Antibody (CAB0471)

Immunohistochemistry of paraffin-embedded human liver cancer using PRKCD antibody (CAB0471) at dilution of 1:100 (40x lens).

Immunohistochemistry - PRKCD Polyclonal Antibody (CAB0471)

Immunohistochemistry of paraffin-embedded human esophagus using PRKCD antibody (CAB0471) at dilution of 1:100 (40x lens).

PRKCD Polyclonal Antibody (CAB0471) Protein Information

UniProt Protein Function:Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Negatively regulates B cell proliferation and also has an important function in self-antigen induced B cell tolerance induction. Upon DNA damage, activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Is highly expressed in a number of cancer cells and promotes cell survival and resistance against chemotherapeutic drugs by inducing cyclin D1 (CCND1) and hyperphosphorylation of RB1, and via several pro-survival pathways, including NF-kappa-B, AKT1 and MAPK1/3 (ERK1/2). Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl-leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion; acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. The catalytic subunit phosphorylates 14-3-3 proteins (YWHAB, YWHAZ and YWHAH) in a sphingosine-dependent fashion. Phosphorylates ELAVL1 in response to angiotensin-2 treatment (PubMed:18285462).
NCBI Summary:Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:Q05655
NCBI GenInfo Identifier:205371776
NCBI Gene ID:5580
NCBI Accession:Q05655.2
UniProt Secondary Accession:Q05655,Q15144, Q86XJ6, B0KZ81, B2R834,
UniProt Related Accession:Q05655
Molecular Weight:80,953 Da
NCBI Full Name:Protein kinase C delta type
NCBI Synonym Full Names:protein kinase C delta
NCBI Official Symbol:PRKCD  
NCBI Official Synonym Symbols:MAY1; PKCD; ALPS3; CVID9; nPKC-delta  
NCBI Protein Information:protein kinase C delta type
UniProt Protein Name:Protein kinase C delta type
UniProt Synonym Protein Names:Tyrosine-protein kinase PRKCD (EC:2.7.10.2); nPKC-delta
Protein Family:Protein kinase
UniProt Gene Name:PRKCD  
UniProt Entry Name:KPCD_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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