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PRKCD Polyclonal Antibody (CAB0471)

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SKU:
CAB0471
  • Western blot - PRKCD Polyclonal Antibody (CAB0471)
  • Immunohistochemistry - PRKCD Polyclonal Antibody (CAB0471)
  • Immunohistochemistry - PRKCD Polyclonal Antibody (CAB0471)
$331.89

Description

Overview

Title:PRKCD Polyclonal Antibody (CAB0471)
Size:100µL
Code:CAB0471
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 477-676 of human PRKCD (NP_997704.1).
GeneID:5580
SwissProt:Q05655
Synonyms:PRKCD; ALPS3; CVID9; MAY1; PKCD; nPKC-delta; protein kinase C delta
Calculated MW:77kDa/80kDa
Observed MW:78kDa/85kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:MCF7, Mouse testis
Cellular Location:Cell membrane, Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus, Peripheral membrane protein, perinuclear region

Antibody Images

Western blot - PRKCD Polyclonal Antibody (CAB0471)

Western blot analysis of extracts of various cell lines, using PRKCD antibody (CAB0471) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - PRKCD Polyclonal Antibody (CAB0471)

Immunohistochemistry of paraffin-embedded human liver cancer using PRKCD antibody (CAB0471) at dilution of 1:100 (40x lens).

Immunohistochemistry - PRKCD Polyclonal Antibody (CAB0471)

Immunohistochemistry of paraffin-embedded human esophagus using PRKCD antibody (CAB0471) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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