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PRKAA1 Polyclonal Antibody (CAB11184)

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CAB11184
  • Western blot - PRKAA1 Polyclonal Antibody (CAB11184)
  • Immunohistochemistry - PRKAA1 Polyclonal Antibody (CAB11184)
  • Immunohistochemistry - PRKAA1 Polyclonal Antibody (CAB11184)
  • Immunofluorescence - PRKAA1 Polyclonal Antibody (CAB11184)
$331.89

Description

Overview

Title:PRKAA1 Polyclonal Antibody (CAB11184)
Size:100µL
Code:CAB11184
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PRKAA1 (NP_006242.5).
GeneID:5562
SwissProt:Q13131
Synonyms:PRKAA1; AMPK; AMPKa1; protein kinase AMP-activated catalytic subunit alpha 1
Calculated MW:64kDa/65kDa
Observed MW:65kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:20 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:293T, HT-29
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - PRKAA1 Polyclonal Antibody (CAB11184)

Western blot analysis of extracts of various cell lines, using PRKAA1 antibody (CAB11184) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - PRKAA1 Polyclonal Antibody (CAB11184)

Immunohistochemistry of paraffin-embedded rat islets of langerhans using PRKAA1 antibody (CAB11184) at dilution of 1:100 (40x lens).

Immunohistochemistry - PRKAA1 Polyclonal Antibody (CAB11184)

Immunohistochemistry of paraffin-embedded human stomach using PRKAA1 antibody (CAB11184) at dilution of 1:100 (40x lens).

Immunofluorescence - PRKAA1 Polyclonal Antibody (CAB11184)

Immunofluorescence analysis of HeLa cells using PRKAA1 antibody (CAB11184).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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