PIK3R1 Polyclonal Antibody (CAB0054)

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CAB0054
  • Western blot - PIK3R1 Polyclonal Antibody (CAB0054)
  • Immunohistochemistry - PIK3R1 Polyclonal Antibody (CAB0054)
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Description

Antibody DatasheetAntibody MSDS

PIK3R1 Polyclonal Antibody (CAB0054)

Overview

Title:PIK3R1 Polyclonal Antibody (CAB0054)
Size:100µL
Code:CAB0054
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PIK3R1 (NP_852556.2).
GeneID:5295
SwissProt:P27986
Synonyms:PIK3R1; AGM7; GRB1; IMD36; p85; p85-ALPHA; phosphoinositide-3-kinase regulatory subunit 1
Calculated MW:42kDa/49kDa/53kDa/83kDa/84kDa
Observed MW:90kDa

Applications

Reactivity:Human, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:THP-1
Cellular Location:N/A

Antibody Images

Western blot - PIK3R1 Polyclonal Antibody (CAB0054)

Western blot analysis of extracts of THP-1 cells, using PIK3R1 antibody (CAB0054) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry - PIK3R1 Polyclonal Antibody (CAB0054)

Immunohistochemistry of paraffin-embedded rat brain using PIK3R1 antibody (CAB0054) at dilution of 1:100 (40x lens).

PIK3R1 Polyclonal Antibody (CAB0054) Protein Information

UniProt Protein Function:PIK3R1: regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Enzyme, regulatory subunit; Kinase, lipid

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: membrane; cytoplasm; plasma membrane; intercellular junction; nucleus; cytosol; phosphoinositide 3-kinase complex

Molecular Function:insulin binding; insulin-like growth factor receptor binding; protein binding; ErbB-3 class receptor binding; insulin receptor substrate binding; neurotrophin TRKA receptor binding; phosphoinositide 3-kinase binding; phosphoinositide 3-kinase regulator activity; transcription factor binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; protein phosphatase binding; insulin receptor binding

Biological Process: negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; viral reproduction; NFAT protein import into nucleus; positive regulation of RNA splicing; regulation of phosphoinositide 3-kinase activity; protein amino acid phosphorylation; T cell receptor signaling pathway; positive regulation of glucose import; phosphoinositide phosphorylation; phospholipid metabolic process; positive regulation of transcription factor import into nucleus; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; protein stabilization; positive regulation of tumor necrosis factor production; negative regulation of cell-matrix adhesion; DNA damage response, signal transduction resulting in induction of apoptosis; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; induction of apoptosis via death domain receptors; B cell differentiation; T cell costimulation; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; positive regulation of cell migration; negative regulation of apoptosis

Disease: Agammaglobulinemia 7, Autosomal Recessive; Short Syndrome; Immunodeficiency 36

NCBI Summary:Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
UniProt Code:P27986
NCBI GenInfo Identifier:118572681
NCBI Gene ID:5295
NCBI Accession:P27986.2
UniProt Secondary Accession:P27986,Q15747, Q4VBZ7, Q53EM6, Q8IXA2, Q8N1C5, B3KT19 D3DWA0, E7EX19,
UniProt Related Accession:P27986
Molecular Weight:42,838 Da
NCBI Full Name:Phosphatidylinositol 3-kinase regulatory subunit alpha
NCBI Synonym Full Names:phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
NCBI Official Symbol:PIK3R1  
NCBI Official Synonym Symbols:p85; AGM7; GRB1; IMD36; p85-ALPHA  
NCBI Protein Information:phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; ptdIns-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit; phosphatidylinositol 3-kinase-associated p-85 alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
UniProt Protein Name:Phosphatidylinositol 3-kinase regulatory subunit alpha
UniProt Synonym Protein Names:Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
UniProt Gene Name:PIK3R1  
UniProt Entry Name:P85A_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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