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PIK3CB Polyclonal Antibody (CAB0982)

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CAB0982
  • Western blot - PIK3CB Polyclonal Antibody (CAB0982)
  • Immunohistochemistry - PIK3CB Polyclonal Antibody (CAB0982)
  • Immunohistochemistry - PIK3CB Polyclonal Antibody (CAB0982)
  • Immunohistochemistry - PIK3CB Polyclonal Antibody (CAB0982)
€299.00

Description

Overview

Title:PIK3CB Polyclonal Antibody (CAB0982)
Size:100µL
Code:CAB0982
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 771-1070 of human PIK3CB (NP_006210.1).
GeneID:5291
SwissProt:P42338
Synonyms:PIK3CB; P110BETA; PI3K; PI3KBETA; PIK3C1; phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit beta
Calculated MW:122kDa
Observed MW:122kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:DU145, SW480, HeLa, Mouse lung
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - PIK3CB Polyclonal Antibody (CAB0982)

Western blot analysis of extracts of various cell lines, using PIK3CB antibody (CAB0982) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

Immunohistochemistry - PIK3CB Polyclonal Antibody (CAB0982)

Immunohistochemistry of paraffin-embedded mouse lung using PIK3CB antibody (CAB0982) at dilution of 1:100 (40x lens).

Immunohistochemistry - PIK3CB Polyclonal Antibody (CAB0982)

Immunohistochemistry of paraffin-embedded human lung using PIK3CB antibody (CAB0982) at dilution of 1:100 (40x lens).

Immunohistochemistry - PIK3CB Polyclonal Antibody (CAB0982)

Immunohistochemistry of paraffin-embedded human colon carcinoma using PIK3CB antibody (CAB0982) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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