The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

Phospho-PRKAA1-S496 pAb (CABP0619)

(No reviews yet) Write a Review
SKU:
CABP0619
€429.00

Description

Overview

Title:Phospho-PRKAA1-S496 pAb (CABP0619)
Size:100µL
Code:CABP0619
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen Information

Immunogen:A phospho specific peptide corresponding to residues surrounding S487 of human PRKAA1
GeneID:5562
SwissProt:Q13131
Synonyms:PRKAA1; AMPK; AMPKa1; protein kinase AMP-activated catalytic subunit alpha 1
Calculated MW:64kDa/65kDa
Observed MW:64kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:1000; IHC 1:50 - 1:100; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HUVEC
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - Phospho-PRKAA1-S496 pAb (CABP0619)

Western blot analysis of extracts of Untreated and treated HUVEC cell lines?using Phospho-PRKAA1-S487 Antibody (CABP0619).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
View AllClose