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Phospho-NPM1-T199 pAb (CABP0836)

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SKU:
CABP0836
€429.00

Description

Overview

Title:Phospho-NPM1-T199 pAb (CABP0836)
Size:100µL
Code:CABP0836
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.

Immunogen Information

Immunogen:A synthetic phosphorylated peptide around T199 of human NPM1 (NP_001032827.1).
GeneID:4869
SwissProt:P06748
Synonyms:NPM1; B23; NPM; nucleophosmin
Calculated MW:28kDa/29kDa/32kDa
Observed MW:38kDa

Applications

Reactivity:Human, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HeLa, PC-12
Cellular Location:Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus, nucleoplasm

Antibody Images

Western blot - Phospho-NPM1-T199 pAb (CABP0836)

Western blot analysis of extracts of various cell lines, using Phospho-NPM1-T199 antibody (CABP0836) at 1:1000 dilution. Hela cells were treated by Nocodazole (100 ng/ml) for 24 hours. PC-12 cells were treated by Nocodazole (1 ?g/ml) for 16 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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