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Phospho-LMNA-S22 pAb (CABP0777)

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CABP0777
  • Western blot - Phospho-LMNA-S22 pAb (CABP0777)
  • Immunoprecipitation - Phospho-LMNA-S22 pAb (CABP0777)
€429.00

Description

Overview

Title:Phospho-LMNA-S22 pAb (CABP0777)
Size:100µL
Code:CABP0777
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Immunogen Information

Immunogen:A synthetic phosphorylated peptide around S22 of human LMNA (NP_005563.1).
GeneID:4000
SwissProt:P02545
Synonyms:LMNA; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1; lamin
Calculated MW:62-74kDa
Observed MW:69kDa, 78kDa

Applications

Reactivity:Human, Rat
Tested Applications:WB; IP
Recommended Dilution:WB 1:500 - 1:2000; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunoprecipitation    
Positive Samples:HeLa, C6
Cellular Location:Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm

Antibody Images

Western blot - Phospho-LMNA-S22 pAb (CABP0777)

Western blot analysis of extracts of various cell lines, using Phospho-LMNA-S22 antibody (CABP0777) at 1:2000 dilution or LMNA antibody (CAB0249). HeLa cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours or treated by Paclitaxel (100 nM) at 37°C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 1s.

Immunoprecipitation - Phospho-LMNA-S22 pAb (CABP0777)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-LMNA-S22 pAb (CABP0777). Western blot was performed from the immunoprecipitate using Phospho-LMNA-S22 pAb (CABP0777) at a dilition of 1:1000. HeLa cells were treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IP
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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