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Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)

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CABP0472
  • Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)
  • Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)
  • Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)
  • Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)
  • Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)
  • Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)
  • Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)
  • Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)
  • Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)
  • Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)
€429.00

Description

Overview

Title:Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)
Size:100µL
Code:CABP0472
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3).
GeneID:5594/5595
SwissProt:P28482/P27361
Synonyms:N/A
Calculated MW:36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW:38kDa, 41kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunoprecipitation    
Positive Samples:Jurkat
Cellular Location:N/A

Antibody Images

Western blot - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)

Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (CABP0472) at 1:5000 dilution or ERK1/2 antibody (CAB16686).293T cells and C6 cells were treated by ATP(5 mM) at 30°C for 1 hour. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)

Immunohistochemistry of paraffin-embedded rat lung using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (CABP0472) at dilution of 1:100 (40x lens).

Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)

Immunohistochemistry of paraffin-embedded rat testis using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (CABP0472) at dilution of 1:100 (40x lens).

Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)

Immunohistochemistry of paraffin-embedded rat brain using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (CABP0472) at dilution of 1:100 (40x lens).

Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)

Immunohistochemistry of paraffin-embedded rat spleen using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (CABP0472) at dilution of 1:100 (40x lens).

Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)

Immunohistochemistry of paraffin-embedded rat heart using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (CABP0472) at dilution of 1:100 (40x lens).

Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)

Immunohistochemistry of paraffin-embedded human tonsil using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (CABP0472) at dilution of 1:100 (40x lens).

Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)

Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (CABP0472) at dilution of 1:100 (40x lens).

Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)

Immunohistochemistry of paraffin-embedded human gastric cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (CABP0472) at dilution of 1:100 (40x lens).

Immunohistochemistry - Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (CABP0472)

Immunohistochemistry of paraffin-embedded human uterine cancer using Phospho-ERK1-T202-Y204 + ERK2-T185-Y187 antibody (CABP0472) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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