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Phospho-EIF2AK2-T446 mAb (CABP0632)

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CABP0632
  • Western blot - Phospho-EIF2AK2-T446 mAb (CABP0632)
  • Immunohistochemistry - Phospho-EIF2AK2-T446 mAb (CABP0632)
$560.55

Description

Overview

Title:Phospho-EIF2AK2-T446 mAb (CABP0632)
Size:100µL
Code:CABP0632
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.

Immunogen Information

Immunogen:A phospho specific peptide corresponding to residues surrounding T446 of human EIF2AK2
GeneID:5610
SwissProt:P19525
Synonyms:EIF2AK2; EIF2AK1; PKR; PPP1R83; PRKR; eukaryotic translation initiation factor 2 alpha kinase 2
Calculated MW:57kDa/62kDa
Observed MW:62kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:HeLa
Cellular Location:Cytoplasm, Nucleus, perinuclear region

Antibody Images

Western blot - Phospho-EIF2AK2-T446 mAb (CABP0632)

Western blot analysis of extracts of HeLa cells, using Phospho-EIF2AK2-T446 antibody (CABP0632).HeLa cells were treated by TNF-? (20 ng/ml) and Calyculin A (100 nM) at 37°C for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.

Immunohistochemistry - Phospho-EIF2AK2-T446 mAb (CABP0632)

Immunohistochemistry of paraffin-embedded human kidney using Phospho-EIF2AK2-T446 antibody (CABP0632) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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