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Phospho-Bad-S112 pAb (CABP0143)

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CABP0143
  • Western blot - Phospho-Bad-S112 pAb (CABP0143)
  • Immunohistochemistry - Phospho-Bad-S112 pAb (CABP0143)
€429.00

Description

Overview

Title:Phospho-Bad-S112 pAb (CABP0143)
Size:100µL
Code:CABP0143
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Immunogen Information

Immunogen:A phospho specific peptide corresponding to residues surrounding S112 of human Bad
GeneID:572
SwissProt:Q92934
Synonyms:BAD; BBC2; BCL2L8; bcl2-associated agonist of cell death
Calculated MW:18kDa
Observed MW:18kDa

Applications

Reactivity:Human, Mouse, Rat, Monkey
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:cos7
Cellular Location:Cytoplasm, Mitochondrion outer membrane

Antibody Images

Western blot - Phospho-Bad-S112 pAb (CABP0143)

Western blot analysis of extracts from cos7 cells using Phospho-Bad-S112 antibody (CABP0143).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.

Immunohistochemistry - Phospho-Bad-S112 pAb (CABP0143)

Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-Bad-S112 antibody (CABP0143).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Monkey
Host Species:
Rabbit
Isotype:
IgG
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