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PEG10 Polyclonal Antibody (CAB2787)

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CAB2787
€299.00

Description

Overview

Title:PEG10 Polyclonal Antibody (CAB2787)
Size:100µL
Code:CAB2787
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This is a paternally expressed imprinted gene that is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation and apoptosis. Overexpression of this gene has been associated with several malignancies, such as hepatocellular carcinoma and B-cell lymphocytic leukemia. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. Additional isoforms resulting from alternatively spliced transcript variants, and use of upstream non-AUG (CUG) start codon have been reported for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human PEG10 (NP_001035242.1).
GeneID:23089
SwissProt:Q86TG7
Synonyms:PEG10; EDR; HB-1; MEF3L; Mar2; Mart2; RGAG3; RTL2; SIRH1; paternally expressed 10
Calculated MW:36kDa/80kDa
Observed MW:55kDa

Applications

Reactivity:Human
Tested Applications:WB
Recommended Dilution:WB 1:200 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:MCF-7, SW620, HepG2, U-251MG
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - PEG10 Polyclonal Antibody (CAB2787)

Western blot analysis of extracts of various cell lines, using PEG10 antibody (CAB2787) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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