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PDF Polyclonal Antibody (CAB10458)

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SKU:
CAB10458
$331.89

Description

Overview

Title:PDF Polyclonal Antibody (CAB10458)
Size:100µL
Code:CAB10458
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-243 of human PDF (NP_071736.1).
GeneID:64146
SwissProt:Q9HBH1
Synonyms:PDF; peptide deformylase, mitochondrial
Calculated MW:27kDa
Observed MW:20kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:MCF-7, SW620, A-549, HepG2, K-562, Mouse heart, Rat heart
Cellular Location:Mitochondrion

Antibody Images

Western blot - PDF Polyclonal Antibody (CAB10458)

Western blot analysis of extracts of various cell lines, using PDF antibody (CAB10458) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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