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PCNA Monoclonal Antibody (CAB9909)

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SKU:
CAB9909
$476.19

Description

Overview

Title:PCNA Monoclonal Antibody (CAB9909)
Size:100µL
Code:CAB9909
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Immunogen Information

Immunogen:Recombinant protein of human PCNA
GeneID:5111
SwissProt:P12004
Synonyms:PCNA; ATLD2; proliferating cell nuclear antigen
Calculated MW:28kDa
Observed MW:37kDa

Applications

Reactivity:Human, Mouse, Rat, Hamster, Monkey
Tested Applications:WB; IHC; ICC; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; ICC 1:50 - 1:200; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    immunocytochemistry    Immunoprecipitation    
Positive Samples:COS-7, HeLa, NIH/3T3
Cellular Location:Nucleus

Antibody Images

Western blot - PCNA Monoclonal Antibody (CAB9909)

Western blot analysis of extracts of various cell lines, using PCNA antibody (CAB9909) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Application:
IHC
Application:
ICC
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Hamster
Reactivity:
Monkey
Host Species:
Mouse
Isotype:
IgG
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