The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

PARP1 Polyclonal Antibody (CAB0010)

(No reviews yet) Write a Review
SKU:
CAB0010
  • Western blot - PARP1 Polyclonal Antibody (CAB0010)
  • Western blot - PARP1 Polyclonal Antibody (CAB0010)
  • Immunofluorescence - PARP1 Polyclonal Antibody (CAB0010)
  • Immunofluorescence - PARP1 Polyclonal Antibody (CAB0010)
$560.55

Description

Overview

Title:PARP1 Polyclonal Antibody (CAB0010)
Size:100µL
Code:CAB0010
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human PARP1 (NP_001609.2).
GeneID:142
SwissProt:P09874
Synonyms:PARP1; ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PPOL; pADPRT-1; poly(ADP-ribose) polymerase 1
Calculated MW:113kDa
Observed MW:116kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:1000 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:Jurkat, HeLa, RAW264.7, C6, Mouse testis, Mouse brain
Cellular Location:Nucleus, nucleolus

Antibody Images

Western blot - PARP1 Polyclonal Antibody (CAB0010)

Western blot analysis of extracts of various cell lines, using PARP1 antibody (CAB0010) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Western blot - PARP1 Polyclonal Antibody (CAB0010)

Western blot analysis of extracts of various cell lines, using PARP1 antibody (CAB0010) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunofluorescence - PARP1 Polyclonal Antibody (CAB0010)

Immunofluorescence analysis of NIH/3T3 cells using PARP1 antibody (CAB0010) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - PARP1 Polyclonal Antibody (CAB0010)

Immunofluorescence analysis of U2OS cells using PARP1 antibody (CAB0010) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
View AllClose