The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

OPTN Polyclonal Antibody (CAB1845)

(No reviews yet) Write a Review
SKU:
CAB1845
$331.89

Description

Overview

Title:OPTN Polyclonal Antibody (CAB1845)
Size:100µL
Code:CAB1845
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-577 of human OPTN (NP_068815.2).
GeneID:10133
SwissProt:Q96CV9
Synonyms:OPTN; ALS12; FIP2; GLC1E; HIP7; HYPL; NRP; TFIIIA-INTP; optineurin
Calculated MW:59kDa/65kDa
Observed MW:78kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HeLa, OVCAR-3, A-549, Mouse liver, Mouse heart
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Recycling endosome, autophagosome, perinuclear region, trans-Golgi network

Antibody Images

Western blot - OPTN Polyclonal Antibody (CAB1845)

Western blot analysis of extracts of various cell lines, using OPTN antibody (CAB1845) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
View AllClose