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OGT Polyclonal Antibody (CAB2214)

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CAB2214
  • Western blot - OGT Polyclonal Antibody (CAB2214)
  • Immunohistochemistry - OGT Polyclonal Antibody (CAB2214)
  • Immunohistochemistry - OGT Polyclonal Antibody (CAB2214)
€299.00

Description

Overview

Title:OGT Polyclonal Antibody (CAB2214)
Size:100µL
Code:CAB2214
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 857-1036 of human OGT (NP_858059.1).
GeneID:8473
SwissProt:O15294
Synonyms:OGT; HINCUT-1; HRNT1; O-GLCNAC; MRX106; OGT1; O-linked N-acetylglucosamine (GlcNAc) transferase
Calculated MW:74kDa/103kDa/115kDa/116kDa
Observed MW:117kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:SW620, K-562, HeLa, BT474, Mouse spleen
Cellular Location:Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus

Antibody Images

Western blot - OGT Polyclonal Antibody (CAB2214)

Western blot analysis of extracts of various cell lines, using OGT antibody (CAB2214) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - OGT Polyclonal Antibody (CAB2214)

Immunohistochemistry of paraffin-embedded rat heart using OGT antibody (CAB2214) at dilution of 1:100 (40x lens).

Immunohistochemistry - OGT Polyclonal Antibody (CAB2214)

Immunohistochemistry of paraffin-embedded human liver injury using OGT antibody (CAB2214) at dilution of 1:100 (40x lens).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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