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NPAS2 Antibody

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SKU:
PACO0029654
  • NPAS2 Antibody
  • Immunofluorescence staining of Hela cells with PACO0029654 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • IHC image of PACO0029654 diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of PACO0029654 diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Western Blot, Positive WB detected in: HepG2 whole cell  lysate, All lanes: NPAS2 antibody at 2.7µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution, Predicted band size: 92 kDa, Observed band size: 92 kDa

Description

Product SKU: PACO0029654
Size: 50ug
Uniprot: Q99743
Aliases: Neuronal PAS domain-containing protein 2 (Neuronal PAS2) (Basic-helix-loop-helix-PAS protein MOP4) (Class E basic helix-loop-helix protein 9) (bHLHe9) (Member of PAS protein 4) (PAS domain-containing protein 4), NPAS2, BHLHE9 MOP4 PASD4
Gene ID: 4862
Gene Symbol: NPAS2
Protein Name: Neuronal PAS domain-containing protein 2
Antibody Type: Polyclonal
Clone ID:  
Application: ELISA, WB, IHC, IF
Reactivity: Human
Host Species: Rabbit
Modifcation:  
Conjugation:  
Isotype: IgG
Immunogen: Recombinant Human Neuronal PAS domain-containing protein 2 protein (644-763AA)
Purification: >95%, Protein G purified
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