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NHLRC1 Polyclonal Antibody (CAB6669)

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CAB6669
  • Western blot - NHLRC1 Polyclonal Antibody (CAB6669)
  • Immunofluorescence - NHLRC1 Polyclonal Antibody (CAB6669)
$331.89

Description

Overview

Title:NHLRC1 Polyclonal Antibody (CAB6669)
Size:100µL
Code:CAB6669
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a single subunit E3 ubiquitin ligase. Laforin is polyubiquitinated by the encoded protein. Defects in this intronless gene lead to an accumulation of laforin and onset of Lafora disease, also known as progressive myoclonic epilepsy type 2 (EPM2).

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NHLRC1 (NP_940988.2).
GeneID:378884
SwissProt:Q6VVB1
Synonyms:NHLRC1; EPM2A; EPM2B; MALIN; bA204B7.2; E3 ubiquitin-protein ligase NHLRC1
Calculated MW:42kDa
Observed MW:45kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:Mouse liver, Mouse brain, Mouse heart, Rat brain, Rat heart, Rat liver
Cellular Location:Endoplasmic reticulum, Nucleus

Antibody Images

Western blot - NHLRC1 Polyclonal Antibody (CAB6669)

Western blot analysis of extracts of various cell lines, using NHLRC1 antibody (CAB6669) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Immunofluorescence - NHLRC1 Polyclonal Antibody (CAB6669)

Immunofluorescence analysis of HeLa cells using NHLRC1 antibody (CAB6669). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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