Mouse CD29 Monoclonal Antibody (Biotin Conjugated) [KMI6](AGEL0053)

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SKU:
AGEL0053
€202.50
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Description

Overview:

Title:Mouse CD29 Monoclonal Antibody (Biotin Conjugated)
Host Species:Rat
Size:100 ug
Concentration:1mg/mL

Background:

The KMI6 antibody reacts with the 130-kDa integrin beta1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin achains. With a1 through a6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with av (CD51), it forms avbeta1 integrin. It also associates with the integrin a7, a8, and a9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including leukocytes, endothelia, smooth muscle, epithelia, and oocytes. The epitope recognized by the KMI6 mAb can be detected by immunoblot in unreduced extracts from a variety of cells and tissues having a number of different beta1-integrin heterodimer combinations. However, the epitope is not well expressed on the surface of the majority of hematopoietic cells. It is detected on the surface of fetal liver cells, some bone marrow stromal cells, and some cultured hematopoietic cell lines, including P815 and PA6. Surface expression of the CD29 epitope recognized by mAb KMI6 on thymocytes and T lymphocytes appears to be developmentally regulated, and KMI6 antibody enhances the adhesion of KMI6 thymocytes to fibronectin and laminin.

Antibody Information:

Isotype:IgG2a
GeneID:16412
SwissProt:P09055
Synonyms:Integrin beta-1, ITB1, Itgb1, Fibronectin receptor subunit beta, VLA-4 subunit beta, CD29
Clone:KMI6
Conjugation:Biotin

Applications:

Reactivity:Mouse
Tested Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ? 2.0 µg per 10e6 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Keep as concentrated solution.Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Reactivity:
Mouse
Host Species:
Rat
Isotype:
IgG2a
Clone:
KMI6
Conjugation:
Biotin
Application:
FC
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