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Mouse anti HA-Tag mAb (CABE008)

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CABE008
  • Western blot - Mouse anti HA-Tag mAb (CABE008)
  • Immunofluorescence - Mouse anti HA-Tag mAb (CABE008)
  • Immunoprecipitation - Mouse anti HA-Tag mAb (CABE008)
$331.89

Description

Overview

Title:Mouse anti HA-Tag mAb (CABE008)
Size:100 uL
Code:CABE008
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:A synthetic peptide of YPYDVPDYA (HA-tag)-KLH
GeneID:N/A
SwissProt:N/A
Synonyms:N/A
Calculated MW:N/A
Observed MW:57kDa

Applications

Reactivity:N/A
Tested Applications:WB; IF; IP
Recommended Dilution:WB 1:2000 - 1:5000; IF 1:50 - 1:500; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    Immunoprecipitation    
Positive Samples:293T
Cellular Location:N/A

Antibody Images

Western blot - Mouse anti HA-Tag mAb (CABE008)

Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody (CABE008) at different dilution. Each lane was loaded with 2 ug cell lysate.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunofluorescence - Mouse anti HA-Tag mAb (CABE008)

Immunofluorescence analysis of 293T cells using Mouse anti HA-Tag mAb (CABE008). Blue: DAPI for nuclear staining.

Immunoprecipitation - Mouse anti HA-Tag mAb (CABE008)

Immunoprecipitation of over-expressed HA-tagged protein in 293T cells incubated with HA-tag antibody (CABE008). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Application:
IF
Application:
IP
Host Species:
Mouse
Isotype:
IgG
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