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Mouse anti GFP-Tag mAb (CABE012)

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CABE012
  • Western blot - Mouse anti GFP-Tag mAb (CABE012)
  • Western blot - Mouse anti GFP-Tag mAb (CABE012)
  • Immunofluorescence - Mouse anti GFP-Tag mAb (CABE012)
  • Immunoprecipitation - Mouse anti GFP-Tag mAb (CABE012)
$442.89

Description

Overview

Title:Mouse anti GFP-Tag mAb (CABE012)
Size:200 uL
Code:CABE012
Host Species:Mouse
Purification:Affinity purification
Isotype:IgG

Background

N/A

Immunogen Information

Immunogen:Recombinant protein of GFP
GeneID:N/A
SwissProt:N/A
Synonyms:N/A
Calculated MW:N/A
Observed MW:55kDa

Applications

Reactivity:N/A
Tested Applications:WB; IF; IP
Recommended Dilution:WB 1:2000 - 1:5000; IF 1:50 - 1:100; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    Immunoprecipitation    
Positive Samples:N/A
Cellular Location:N/A

Antibody Images

Western blot - Mouse anti GFP-Tag mAb (CABE012)

Western blot analysis of over-expressed GFP fusion protein in 293 cell using GFP-Tag antibody (CABE012) at different dilution. Each lane was loaded with 2 ug cell lysate.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - Mouse anti GFP-Tag mAb (CABE012)

Western blot analysis of over-expressed GFP fusion protein in 293T cell using GFP-Tag antibody (CABE012) at different dilution. Each lane was loaded with 2 ug cell lysate.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunofluorescence - Mouse anti GFP-Tag mAb (CABE012)

Immunofluorescence analysis of 293T cells using Mouse anti GFP-Tag mAb (CABE012). Blue: DAPI for nuclear staining.

Immunoprecipitation - Mouse anti GFP-Tag mAb (CABE012)

Immunoprecipitation of over-expressed GFP-tagged protein in 293T cells incubated using GFP-tag antibody (CABE012). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Application:
IF
Application:
IP
Host Species:
Mouse
Isotype:
IgG
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