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Mouse anti DDDDK-Tag mAb (CABE005)

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CABE005
  • Western blot - Mouse anti DDDDK-Tag mAb (CABE005)
  • Immunofluorescence - Mouse anti DDDDK-Tag mAb (CABE005)
  • Immunoprecipitation - Mouse anti DDDDK-Tag mAb (CABE005)
€329.00

Description

Overview

Title: Mouse anti DDDDK-Tag mAb (CABE005)
Size: 100µL
Code: CABE005
Host Species: Mouse
Purification: Affinity purification
Isotype: IgG  

Background

N/A

Immunogen Information

Immunogen: A synthetic Peptide of human DDDDK-Tag
GeneID:  

Applications

Reactivity: Human, Mouse
Tested Applications: WB, IHC, IF, IP
Recommended Dilution: WB 1:2000 - 1:5000 IHC 1:50 - 1:500 IF 1:50 - 1:500 IP 1:50 - 1:100
Storage Buffer: Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Positive Samples: 293T

Antibody Images

Western blot - Mouse anti DDDDK-Tag mAb (CABE005)

Western blot analysis of over-expressed DDDDK-tagged protein in 293T cells using DDDDK-tag antibody (CABE005) at different dilution.Each lane was loaded with 2 ug cell lysate.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Immunofluorescence - Mouse anti DDDDK-Tag mAb (CABE005)

Immunofluorescence analysis of 293T cells using Mouse anti DDDDK-Tag mAb (CABE005). Blue: DAPI for nuclear staining.

Immunoprecipitation - Mouse anti DDDDK-Tag mAb (CABE005)

Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated with DDDDK-tag antibody (CABE005). A mock served as negative control and over-expressed 293T cell lysate served as positive control.

Datasheets

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