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MAP1LC3B Polyclonal Antibody (CAB15591)

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SKU:
CAB15591
  • Western blot - MAP1LC3B Polyclonal Antibody (CAB15591)
  • Immunohistochemistry - MAP1LC3B Polyclonal Antibody (CAB15591)
  • Immunohistochemistry - MAP1LC3B Polyclonal Antibody (CAB15591)
  • Immunohistochemistry - MAP1LC3B Polyclonal Antibody (CAB15591)
  • Immunofluorescence - MAP1LC3B Polyclonal Antibody (CAB15591)
€299.00

Description

Overview

Title:MAP1LC3B Polyclonal Antibody (CAB15591)
Size:100µL
Code:CAB15591
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human MAP1LC3B (NP_073729.1).
GeneID:81631
SwissProt:Q9GZQ8
Synonyms:MAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta
Calculated MW:14kDa
Observed MW:14kDa, 16kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, NIH/3T3
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton

Antibody Images

Western blot - MAP1LC3B Polyclonal Antibody (CAB15591)

Western blot analysis of extracts of various cell lines, using MAP1LC3B antibody (CAB15591) at 1:1000 dilution. Hela cells were treated by Chloroquine (50 ?M) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 ?M) for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.

Immunohistochemistry - MAP1LC3B Polyclonal Antibody (CAB15591)

Immunohistochemistry of paraffin-embedded human breast cancer using MAP1LC3B antibody (CAB15591) at dilution of 1:100 (40x lens).

Immunohistochemistry - MAP1LC3B Polyclonal Antibody (CAB15591)

Immunohistochemistry of paraffin-embedded human gastric cancer using MAP1LC3B antibody (CAB15591) at dilution of 1:100 (40x lens).

Immunohistochemistry - MAP1LC3B Polyclonal Antibody (CAB15591)

Immunohistochemistry of paraffin-embedded human mammary cancer using MAP1LC3B antibody (CAB15591) at dilution of 1:100 (40x lens).

Immunofluorescence - MAP1LC3B Polyclonal Antibody (CAB15591)

Immunofluorescence analysis of HeLa cells using MAP1LC3B antibody (CAB15591) at dilution of 1:100. Hela cells were treated by Chloroquine (50 ?M) for 20 hours(left). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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