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MAP1LC3B Polyclonal Antibody (CAB11282)

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CAB11282
  • Western blot - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)
  • Western blot - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)
  • Immunohistochemistry - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)
  • Immunohistochemistry - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)
  • Immunohistochemistry - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)
  • Immunohistochemistry - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)
  • Immunofluorescence - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)
  • Immunofluorescence - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)
$331.89

Description

Overview

Title:MAP1LC3B Polyclonal Antibody (CAB11282)
Size:100µL
Code:CAB11282
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Immunogen Information

Immunogen:A synthetic peptide of human MAP1LC3B
GeneID:81631
SwissProt:Q9GZQ8
Synonyms:MAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta
Calculated MW:14kDa
Observed MW:14kDa, 16kDa

Applications

Reactivity:Human, Mouse, Rat, Pig
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:HeLa, NIH/3T3
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton

Antibody Images

Western blot - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)

Western blot analysis of extracts of various cell lines, using LC3B antibody (CAB11282) at 1:1000 dilution. Both C6 cells and NIH/3T3 cells were treated by Chloroquine (50 ?M) at 37°C for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

Western blot - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)

Western blot analysis of extracts from normal (control) and MAP1LC3B knockout (KO) 293T cells, using MAP1LC3B antibody (CAB11282) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)

Immunohistochemistry of paraffin-embedded rat lung using MAP1LC3B antibody (CAB11282) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)

Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3B antibody (CAB11282) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)

Immunohistochemistry of paraffin-embedded human lung cancer using MAP1LC3B antibody (CAB11282) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)

Immunohistochemistry of paraffin-embedded mouse testis using MAP1LC3B antibody (CAB11282) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)

Immunofluorescence analysis of HeLa cells using MAP1LC3B antibody (CAB11282) at dilution of 1:100. Hela cells were treated by Chloroquine (50 ?M) for 20 hours(left). Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] MAP1LC3B Polyclonal Antibody (CAB11282)

Immunofluorescence analysis of NIH/3T3 cells using MAP1LC3B antibody (CAB11282) at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 ?M) for 20 hours(left). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Pig
Host Species:
Rabbit
Isotype:
IgG
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