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MAP1LC3A Polyclonal Antibody (CAB5618)

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CAB5618
  • Western blot - MAP1LC3A Polyclonal Antibody (CAB5618)
  • Immunofluorescence - MAP1LC3A Polyclonal Antibody (CAB5618)
£254.15

Description

Overview

Title:MAP1LC3A Polyclonal Antibody (CAB5618)
Size:100µL
Code:CAB5618
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-121 of human MAP1LC3A (NP_115903.1).
GeneID:84557
SwissProt:Q9H492
Synonyms:MAP1LC3A; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3; microtubule-associated proteins 1A/1B light chain 3A
Calculated MW:14kDa
Observed MW:14kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:293T, SH-SY5Y, A375, Mouse brain, Rat brain
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton

Antibody Images

Western blot - MAP1LC3A Polyclonal Antibody (CAB5618)

Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody (CAB5618) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.

Immunofluorescence - MAP1LC3A Polyclonal Antibody (CAB5618)

Immunofluorescence analysis of HeLa cells using MAP1LC3A antibody (CAB5618).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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