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MAP1LC3A Monoclonal Antibody (CAB11428)

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SKU:
CAB11428
  • Western blot - MAP1LC3A Monoclonal Antibody (CAB11428)
  • Immunohistochemistry - MAP1LC3A Monoclonal Antibody (CAB11428)
  • Immunohistochemistry - MAP1LC3A Monoclonal Antibody (CAB11428)
  • Immunohistochemistry - MAP1LC3A Monoclonal Antibody (CAB11428)
  • Immunofluorescence - MAP1LC3A Monoclonal Antibody (CAB11428)
  • Immunofluorescence - MAP1LC3A Monoclonal Antibody (CAB11428)
  • Immunofluorescence - MAP1LC3A Monoclonal Antibody (CAB11428)
€429.00

Description

Overview

Title:MAP1LC3A Monoclonal Antibody (CAB11428)
Size:100µL
Code:CAB11428
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

Immunogen Information

Immunogen:Recombinant protein of human MAP1LC3A
GeneID:84557
SwissProt:Q9H492
Synonyms:MAP1LC3A; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3; microtubule-associated proteins 1A/1B light chain 3A
Calculated MW:14kDa
Observed MW:14kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:SHG-44, Mouse brain, Mouse liver, Mouse skeletal muscle
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton

Antibody Images

Western blot - MAP1LC3A Monoclonal Antibody (CAB11428)

Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody (CAB11428).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry - MAP1LC3A Monoclonal Antibody (CAB11428)

Immunohistochemistry of paraffin-embedded mouse brain using MAP1LC3A antibody (CAB11428) at dilution of 1:100 (40x lens).

Immunohistochemistry - MAP1LC3A Monoclonal Antibody (CAB11428)

Immunohistochemistry of paraffin-embedded human liver using MAP1LC3A antibody (CAB11428) at dilution of 1:100 (40x lens).

Immunohistochemistry - MAP1LC3A Monoclonal Antibody (CAB11428)

Immunohistochemistry of paraffin-embedded mouse liver using MAP1LC3A antibody (CAB11428) at dilution of 1:100 (40x lens).

Immunofluorescence - MAP1LC3A Monoclonal Antibody (CAB11428)

Immunofluorescence analysis of HeLa cells using MAP1LC3A antibody (CAB11428).

Immunofluorescence - MAP1LC3A Monoclonal Antibody (CAB11428)

Immunofluorescence analysis of PC-12 cells using MAP1LC3A antibody (CAB11428).

Immunofluorescence - MAP1LC3A Monoclonal Antibody (CAB11428)

Immunofluorescence analysis of HUVEC cells using MAP1LC3A antibody (CAB11428).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Monoclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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