MAML1 Polyclonal Antibody (CAB17060)

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CAB17060
€299.00
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Description

Antibody DatasheetAntibody MSDS

MAML1 Polyclonal Antibody (CAB17060)

Overview

Title:MAML1 Polyclonal Antibody (CAB17060)
Size:100µL
Code:CAB17060
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway.

Immunogen Information

Immunogen:Recombinant protein of human MAML1.
GeneID:9794
SwissProt:Q92585
Synonyms:MAML1; Mam-1; Mam1; mastermind-like protein 1
Calculated MW:108kDa
Observed MW:120kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HeLa, Jurkat, mouse brain, rat brain
Cellular Location:N/A

Antibody Images

Western blot - MAML1 Polyclonal Antibody (CAB17060)

Western blot analysis of extracts of various cell lines, using MAML1 antibody (CAB17060) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

MAML1 Polyclonal Antibody (CAB17060) Protein Information

UniProt Protein Function:MAML1: Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. Interacts (via N-terminus) with NOTCH1, NOTCH2, NOTCH3 and NOTCH4 (via ankyrin repeat region). Interacts (via N-terminus) with p53 (via DNA-binding region). Forms a DNA-binding complex with Notch proteins and RBPSUH/RBP-J kappa/CBF1. Also binds CREBBP/CBP and CDK8. Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen. Belongs to the mastermind family.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function:peptide antigen binding; protein binding; protein kinase binding; transcription coactivator activity

Biological Process: activation of Notch receptor target transcription factor; Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; transcription initiation from RNA polymerase II promoter

NCBI Summary:This protein is the human homolog of mastermind, a Drosophila protein that plays a role in the Notch signaling pathway involved in cell-fate determination. There is in vitro evidence that the human homolog forms a complex with the intracellular portion of human Notch receptors and can increase expression of a Notch-induced gene. This evidence supports its proposed function as a transcriptional co-activator in the Notch signaling pathway. [provided by RefSeq, Jul 2008]
UniProt Code:Q92585
NCBI GenInfo Identifier:68565602
NCBI Gene ID:9794
NCBI Accession:Q92585.3
UniProt Secondary Accession:Q92585,Q9NZ12,
UniProt Related Accession:Q92585
Molecular Weight:108,054 Da
NCBI Full Name:Mastermind-like protein 1
NCBI Synonym Full Names:mastermind like transcriptional coactivator 1
NCBI Official Symbol:MAML1  
NCBI Official Synonym Symbols:Mam1; Mam-1  
NCBI Protein Information:mastermind-like protein 1
UniProt Protein Name:Mastermind-like protein 1
UniProt Gene Name:MAML1  
UniProt Entry Name:MAML1_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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