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Galectin-8 Polyclonal Antibody (CAB6646)

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SKU:
CAB6646
  • Western blot - LGALS8 Polyclonal Antibody (CAB6646)
  • Immunohistochemistry - LGALS8 Polyclonal Antibody (CAB6646)
  • Immunohistochemistry - LGALS8 Polyclonal Antibody (CAB6646)
  • Immunofluorescence - LGALS8 Polyclonal Antibody (CAB6646)
€299.00

Description

Overview

Title:LGALS8 Polyclonal Antibody (CAB6646)
Size:100µL
Code:CAB6646
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-317 of human LGALS8 (NP_963838.1).
GeneID:3964
SwissProt:O00214
Synonyms:LGALS8; Gal-8; PCTA-1; PCTA1; Po66-CBP; galectin-8
Calculated MW:35kDa/40kDa
Observed MW:38kDa, 50kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:10 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:BT-474, 22Rv1, Mouse liver, Mouse small intestine
Cellular Location:Cytoplasm

Antibody Images

Western blot - LGALS8 Polyclonal Antibody (CAB6646)

Western blot analysis of extracts of various cell lines, using LGALS8 antibody (CAB6646) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Immunohistochemistry - LGALS8 Polyclonal Antibody (CAB6646)

Immunohistochemistry of paraffin-embedded human colon carcinoma using LGALS8 antibody (CAB6646) at dilution of 1:100 (40x lens).

Immunohistochemistry - LGALS8 Polyclonal Antibody (CAB6646)

Immunohistochemistry of paraffin-embedded human gastric cancer using LGALS8 antibody (CAB6646) at dilution of 1:100 (40x lens).

Immunofluorescence - LGALS8 Polyclonal Antibody (CAB6646)

Immunofluorescence analysis of A549 cells using LGALS8 antibody (CAB6646). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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