LEFTY1 Polyclonal Antibody (CAB15389)

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CAB15389
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Description

Antibody DatasheetAntibody MSDS

LEFTY1 Polyclonal Antibody (CAB15389)

Overview

Title:LEFTY1 Polyclonal Antibody (CAB15389)
Size:100µL
Code:CAB15389
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This gene is closely linked to both a related family member and a related pseudogene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 77-366 of human LEFTY1 (NP_066277.1).
GeneID:10637
SwissProt:O75610
Synonyms:LEFTY1; LEFTB; LEFTYB; left-right determination factor 1
Calculated MW:40kDa
Observed MW:39kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:200 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HT-29, SW480, BxPC-3, Mouse pancreas, Mouse spleen, Rat pancreas
Cellular Location:Secreted

Antibody Images

Western blot - LEFTY1 Polyclonal Antibody (CAB15389)

Western blot analysis of extracts of various cell lines, using LEFTY1 antibody (CAB15389) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

LEFTY1 Polyclonal Antibody (CAB15389) Protein Information

UniProt Protein Function:LEFTY1: Required for left-right axis determination as a regulator of LEFTY2 and NODAL. Belongs to the TGF-beta family.
UniProt Protein Details:

Protein type:Cytokine; Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q42.1

Cellular Component: extracellular space

Molecular Function:cytokine activity; transforming growth factor beta receptor binding

Biological Process: cell development; determination of left/right symmetry; heart morphogenesis; negative regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; regulation of MAPKKK cascade; transforming growth factor beta receptor signaling pathway

NCBI Summary:This gene encodes a member of the TGF-beta family of proteins. A similar secreted protein in mouse plays a role in left-right asymmetry determination of organ systems during development. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene. [provided by RefSeq, Jul 2008]
UniProt Code:O75610
NCBI GenInfo Identifier:13124321
NCBI Gene ID:10637
NCBI Accession:O75610.1
UniProt Secondary Accession:O75610,Q53H67, Q5TE94, B2R7U0,
UniProt Related Accession:O75610
Molecular Weight:40,880 Da
NCBI Full Name:Left-right determination factor 1
NCBI Synonym Full Names:left-right determination factor 1
NCBI Official Symbol:LEFTY1  
NCBI Official Synonym Symbols:LEFTB; LEFTYB  
NCBI Protein Information:left-right determination factor 1
UniProt Protein Name:Left-right determination factor 1
UniProt Synonym Protein Names:Left-right determination factor B; Protein lefty-1; Protein lefty-B
Protein Family:Left-right determination factor
UniProt Gene Name:LEFTY1  
UniProt Entry Name:LFTY1_HUMAN
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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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