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LAMC1 Polyclonal Antibody (CAB16020)

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CAB16020
  • Western blot - LAMC1 Polyclonal Antibody (CAB16020)
  • Immunofluorescence - LAMC1 Polyclonal Antibody (CAB16020)
  • Immunofluorescence - LAMC1 Polyclonal Antibody (CAB16020)
$331.89

Description

Overview

Title:LAMC1 Polyclonal Antibody (CAB16020)
Size:100µL
Code:CAB16020
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis.

Immunogen Information

Immunogen:Recombinant protein of human LAMC1
GeneID:3915
SwissProt:P11047
Synonyms:LAMC1; LAMB2; laminin subunit gamma-1
Calculated MW:177kDa
Observed MW:240kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:LO2, Mouse heart, Mouse kidney, Rat heart
Cellular Location:N/A

Antibody Images

Western blot - LAMC1 Polyclonal Antibody (CAB16020)

Western blot analysis of extracts of various cell lines, using LAMC1 antibody (CAB16020) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.

Immunofluorescence - LAMC1 Polyclonal Antibody (CAB16020)

Immunofluorescence analysis of rat brain using LAMC1 antibody (CAB16020) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - LAMC1 Polyclonal Antibody (CAB16020)

Immunofluorescence analysis of rat heart using LAMC1 antibody (CAB16020) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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