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[KO Validated] YBX1 Polyclonal Antibody (CAB7704)

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CAB7704
  • Western blot - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)
  • Western blot - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)
  • Immunohistochemistry - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)
  • Immunohistochemistry - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)
  • Immunohistochemistry - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)
  • Immunofluorescence - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)
  • Immunofluorescence - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)
  • Immunofluorescence - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)
  • Immunofluorescence - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)
$553.89

Description

Overview

Title:[KO Validated] YBX1 Polyclonal Antibody (CAB7704)
Size:100µL
Code:CAB7704
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2).
GeneID:4904
SwissProt:P67809
Synonyms:YBX1; BP-8; CBF-A; CSDA2; CSDB; DBPB; EFI-A; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1; Y-box binding protein 1
Calculated MW:35kDa
Observed MW:49kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:100 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:THP-1, Mouse heart, Rat heart
Cellular Location:Cytoplasm, Cytoplasmic granule, Nucleus, Secreted

Antibody Images

Western blot - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)

Western blot analysis of extracts of various cell lines, using YBX1 antibody (CAB7704) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)

Western blot analysis of extracts from normal (control) and YBX1 knockout (KO) HeLa cells, using YBX1 antibody (CAB7704) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Immunohistochemistry - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)

Immunohistochemistry of paraffin-embedded human lung cancer using YBX1 antibody (CAB7704) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)

Immunohistochemistry of paraffin-embedded human prostate using YBX1 antibody (CAB7704) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)

Immunohistochemistry of paraffin-embedded mouse brain using YBX1 antibody (CAB7704) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)

Immunofluorescence analysis of U2OS cells using YBX1 antibody (CAB7704) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)

Immunofluorescence analysis of HeLa cells using YBX1 antibody (CAB7704) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)

Immunofluorescence analysis of NIH/3T3 cells using YBX1 antibody (CAB7704) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - [KO Validated] YBX1 Polyclonal Antibody (CAB7704)

Immunofluorescence analysis of PC-12 cells using YBX1 antibody (CAB7704) at dilution of 1:100. Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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