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[KO Validated] XPA Polyclonal Antibody (CAB1626)

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CAB1626
  • Western blot - [KO Validated] XPA Polyclonal Antibody (CAB1626)
  • Western blot - [KO Validated] XPA Polyclonal Antibody (CAB1626)
  • Immunofluorescence - [KO Validated] XPA Polyclonal Antibody (CAB1626)
  • Immunofluorescence - [KO Validated] XPA Polyclonal Antibody (CAB1626)
$553.89

Description

Overview

Title:[KO Validated] XPA Polyclonal Antibody (CAB1626)
Size:100µL
Code:CAB1626
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human XPA (NP_000371.1).
GeneID:7507
SwissProt:P23025
Synonyms:XPA; XP1; XPAC; DNA repair protein complementing XP-A cells
Calculated MW:31kDa
Observed MW:37kDa

Applications

Reactivity:Human, Mouse
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:HeLa, Jurkat, K-562, Mouse liver, Mouse kidney
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] XPA Polyclonal Antibody (CAB1626)

Western blot analysis of extracts of various cell lines, using XPA antibody (CAB1626) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] XPA Polyclonal Antibody (CAB1626)

Western blot analysis of extracts from normal (control) and XPA knockout (KO) HeLa cells, using XPA antibody (CAB1626) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Immunofluorescence - [KO Validated] XPA Polyclonal Antibody (CAB1626)

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using XPA antibody (CAB1626). Green?GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Immunofluorescence - [KO Validated] XPA Polyclonal Antibody (CAB1626)

Immunofluorescence analysis of A549 cells using XPA antibody (CAB1626).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
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