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[KO Validated] XIAP Polyclonal Antibody (CAB6869)

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SKU:
CAB6869
  • Western blot - [KO Validated] XIAP Polyclonal Antibody (CAB6869)
  • Western blot - [KO Validated] XIAP Polyclonal Antibody (CAB6869)
  • Immunohistochemistry - [KO Validated] XIAP Polyclonal Antibody (CAB6869)
  • Immunohistochemistry - [KO Validated] XIAP Polyclonal Antibody (CAB6869)
  • Immunofluorescence - [KO Validated] XIAP Polyclonal Antibody (CAB6869)
€499.00

Description

Overview

Title:[KO Validated] XIAP Polyclonal Antibody (CAB6869)
Size:100µL
Code:CAB6869
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human XIAP (NP_001158.2).
GeneID:331
SwissProt:P98170
Synonyms:XIAP; API3; BIRC4; IAP-3; ILP1; MIHA; XLP2; hIAP-3; hIAP3; X-linked inhibitor of apoptosis
Calculated MW:56kDa
Observed MW:54kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100; IF 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:MCF-7, HeLa, SW620, HepG2, Jurkat, Mouse liver, Rat kidney
Cellular Location:Cytoplasm, Nucleus

Antibody Images

Western blot - [KO Validated] XIAP Polyclonal Antibody (CAB6869)

Western blot analysis of extracts of various cell lines, using XIAP antibody (CAB6869) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Western blot - [KO Validated] XIAP Polyclonal Antibody (CAB6869)

Western blot analysis of extracts from normal (control) and XIAP knockout (KO) HeLa cells, using XIAP antibody (CAB6869) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Immunohistochemistry - [KO Validated] XIAP Polyclonal Antibody (CAB6869)

Immunohistochemistry of paraffin-embedded human kidney using XIAP antibody (CAB6869) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] XIAP Polyclonal Antibody (CAB6869)

Immunohistochemistry of paraffin-embedded mouse kidney using XIAP antibody (CAB6869) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] XIAP Polyclonal Antibody (CAB6869)

Immunofluorescence analysis of MCF7 cells using XIAP antibody (CAB6869).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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