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[KO Validated] TRAF2 Polyclonal Antibody (CAB0962)

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CAB0962
  • Western blot - [KO Validated] TRAF2 Polyclonal Antibody (CAB0962)
  • Western blot - [KO Validated] TRAF2 Polyclonal Antibody (CAB0962)
  • Immunofluorescence - [KO Validated] TRAF2 Polyclonal Antibody (CAB0962)
£424.15

Description

Overview

Title:[KO Validated] TRAF2 Polyclonal Antibody (CAB0962)
Size:100µL
Code:CAB0962
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF2 (NP_066961.2).
GeneID:7186
SwissProt:Q12933
Synonyms:TRAF2; MGC:45012; TRAP; TRAP3; TNF receptor-associated factor 2
Calculated MW:53kDa/54kDa/55kDa/61kDa
Observed MW:54kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:100; IP 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    Immunoprecipitation    
Positive Samples:HeLa, 293T, THP-1, PC-12, Ramos, Jurkat, NIH/3T3
Cellular Location:Cytoplasm

Antibody Images

Western blot - [KO Validated] TRAF2 Polyclonal Antibody (CAB0962)

Western blot analysis of extracts of various cell lines, using TRAF2 antibody (CAB0962) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western blot - [KO Validated] TRAF2 Polyclonal Antibody (CAB0962)

Western blot analysis of extracts from normal (control) and TRAF2 knockout (KO) 293T cells, using TRAF2 antibody (CAB0962) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 6s.

Immunofluorescence - [KO Validated] TRAF2 Polyclonal Antibody (CAB0962)

Immunofluorescence analysis of HeLa cells using TRAF2 antibody (CAB0962). Blue: DAPI for nuclear staining.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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