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[KO Validated] TMPO Polyclonal Antibody (CAB2534)

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CAB2534
  • Western blot - [KO Validated] TMPO Polyclonal Antibody (CAB2534)
  • Western blot - [KO Validated] TMPO Polyclonal Antibody (CAB2534)
  • Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (CAB2534)
  • Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (CAB2534)
  • Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (CAB2534)
  • Immunofluorescence - [KO Validated] TMPO Polyclonal Antibody (CAB2534)
$553.89

Description

Overview

Title:[KO Validated] TMPO Polyclonal Antibody (CAB2534)
Size:100µL
Code:CAB2534
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).
GeneID:7112
SwissProt:P42166/P42167
Synonyms:TMPO; CMD1T; LAP2; LEMD4; PRO0868; TP; thymopoietin
Calculated MW:38kDa/50kDa/75kDa
Observed MW:56kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    
Positive Samples:22Rv1, K-562, HepG2, 293T, SH-SY5Y, H460
Cellular Location:Chromosome, Nucleus

Antibody Images

Western blot - [KO Validated] TMPO Polyclonal Antibody (CAB2534)

Western blot analysis of extracts of various cell lines, using TMPO antibody (CAB2534) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Western blot - [KO Validated] TMPO Polyclonal Antibody (CAB2534)

Western blot analysis of extracts from normal (control) and TMPO knockout (KO) 293T cells, using TMPO antibody (CAB2534) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (CAB2534)

Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody (CAB2534) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (CAB2534)

Immunohistochemistry of paraffin-embedded human tonsil using TMPO Antibody (CAB2534) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (CAB2534)

Immunohistochemistry of paraffin-embedded mouse brain using TMPO Antibody (CAB2534) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] TMPO Polyclonal Antibody (CAB2534)

Immunofluorescence analysis of U2OS cells using TMPO antibody (CAB2534).

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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