The cookie settings on this website are set to 'allow all cookies' to give you the very best experience. Please click Accept Cookies to continue to use the site.

[KO Validated] TBP Polyclonal Antibody (CAB2192)

(No reviews yet) Write a Review
SKU:
CAB2192
  • Western blot - [KO Validated] TBP Polyclonal Antibody (CAB2192)
  • Western blot - [KO Validated] TBP Polyclonal Antibody (CAB2192)
$553.89

Description

Overview

Title:[KO Validated] TBP Polyclonal Antibody (CAB2192)
Size:100µL
Code:CAB2192
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TBP (NP_001165556.1).
GeneID:6908
SwissProt:P20226
Synonyms:TBP; GTF2D; GTF2D1; HDL4; SCA17; TFIID; TATA-box-binding protein
Calculated MW:35kDa/37kDa
Observed MW:43kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    
Positive Samples:HeLa, MCF-7, K-562, 293T
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] TBP Polyclonal Antibody (CAB2192)

Western blot analysis of extracts of various cell lines, using TBP antibody (CAB2192) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

Western blot - [KO Validated] TBP Polyclonal Antibody (CAB2192)

Western blot analysis of extracts from normal (control) and TBP knockout (KO) HeLa cells, using TBP antibody (CAB2192) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.

Datasheets

Antibody DatasheetAntibody MSDS

View AllClose

Additional Information

Antibody Type:
Polyclonal
Application:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
View AllClose