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[KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)

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CAB13353
  • Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)
  • Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)
  • Immunofluorescence - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)
  • Immunoprecipitation - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)
$553.89

Description

Overview

Title:[KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)
Size:100µL
Code:CAB13353
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3).
GeneID:6605
SwissProt:Q969G3
Synonyms:SMARCE1; BAF57; CSS5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Calculated MW:34kDa/37kDa/39kDa/41kDa/42kDa/46kDa
Observed MW:55kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:U-251MG, MCF-7
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)

Western blot analysis of extracts of various cell lines, using SMARCE1 antibody (CAB13353) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)

Western blot analysis of extracts from normal (control) and SMARCE1 knockout (KO) HeLa cells, using SMARCE1 antibody (CAB13353) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Immunofluorescence - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)

Immunofluorescence analysis of HeLa cells using SMARCE1 antibody (CAB13353).

Immunoprecipitation - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)

Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SMARCE1 antibody (CAB13353). Western blot was performed from the immunoprecipitate using SMARCE1 antibody (CAB13353) at a dilition of 1:1000.

Datasheets

Antibody DatasheetAntibody MSDS

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Additional Information

Antibody Type:
Polyclonal
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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