[KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)

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CAB13353
  • Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)
  • Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)
  • Immunofluorescence - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)
  • Immunoprecipitation - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)
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Description

Antibody DatasheetAntibody MSDS

[KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)

Overview

Title:[KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)
Size:100µL
Code:CAB13353
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3).
GeneID:6605
SwissProt:Q969G3
Synonyms:SMARCE1; BAF57; CSS5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Calculated MW:34kDa/37kDa/39kDa/41kDa/42kDa/46kDa
Observed MW:55kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IHC; IF; IP
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:20 - 1:50
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive Samples:U-251MG, MCF-7
Cellular Location:Nucleus

Antibody Images

Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)

Western blot analysis of extracts of various cell lines, using SMARCE1 antibody (CAB13353) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)

Western blot analysis of extracts from normal (control) and SMARCE1 knockout (KO) HeLa cells, using SMARCE1 antibody (CAB13353) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

Immunofluorescence - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)

Immunofluorescence analysis of HeLa cells using SMARCE1 antibody (CAB13353).

Immunoprecipitation - [KO Validated] SMARCE1 Polyclonal Antibody (CAB13353)

Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SMARCE1 antibody (CAB13353). Western blot was performed from the immunoprecipitate using SMARCE1 antibody (CAB13353) at a dilition of 1:1000.

[KO Validated] SMARCE1 Polyclonal Antibody (CAB13353) Protein Information

UniProt Protein Function:SMARCE1: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Defects in SMARCE1 may be a cause of Coffin-Siris syndrome, a highly variable disease characterized by mental retardation associated with a broad spectrum of different clinical features. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleoplasm; nuclear chromosome; SWI/SNF complex; protein complex; transcriptional repressor complex; nuclear chromatin; nucleus

Molecular Function:ligand-dependent nuclear receptor binding; N-acetyltransferase activity; protein binding; nucleosomal DNA binding; RNA binding; transcription coactivator activity; protein N-terminus binding; chromatin binding

Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase II promoter; nervous system development; nucleosome disassembly; metabolic process; ATP-dependent chromatin remodeling; negative regulation of transcription, DNA-dependent

Disease: Meningioma, Familial, Susceptibility To

NCBI Summary:The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]
UniProt Code:Q969G3
NCBI GenInfo Identifier:61247587
NCBI Gene ID:6605
NCBI Accession:Q969G3.2
UniProt Secondary Accession:Q969G3,O43539, B3KMC1, B4DFR4, C0IMW4, C0IMW5, C0IMW7 H7C3F6,
UniProt Related Accession:Q969G3
Molecular Weight:411
NCBI Full Name:SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
NCBI Synonym Full Names:SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
NCBI Official Symbol:SMARCE1  
NCBI Official Synonym Symbols:BAF57  
NCBI Protein Information:SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1; BRG1-associated factor 57; chromatin remodeling complex BRG1-associated factor 57; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
UniProt Protein Name:SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
UniProt Synonym Protein Names:BRG1-associated factor 57; BAF57
Protein Family:SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily
UniProt Gene Name:SMARCE1  
UniProt Entry Name:SMCE1_HUMAN
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Additional Information

Product type:
Antibody
Application:
WB
Application:
IHC
Application:
IF
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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